ABSTRACT
Cholangiocarcinoma is a group of highly invasive and heterogeneous biliary malignancies originating from any part of the biliary tree. At present, the most ideal treatment is still radical surgery.Gemcitabine combined with cisplatin (gem-cis) has been recognized as the standard first-line chemotherapy regimen for patients with unresectable, advanced or metastatic disease.In recent years, with the proposal of precision medicine and the development of next-generation sequencing technologies, A large number of important cholangiocarcinoma targets have been discovered, such as FGFR, IDH, VEGFR, BRAF, MET, etc., and the research on corresponding target drugs is booming.By referring to relevant literature and data, combined with domestic and foreign clinical trials, this paper reviews the important targets of cholangiocarcinoma and the latest progress of targeted drug therapy.
ABSTRACT
Liver is the most important metastatic target organ of colorectal cancer. Nearly 50% of colorectal patients are found to have liver metastasis during the course of the disease, including 25% of colorectal patients undergoing simultaneous liver metastasis and the other 25% undergoing metachronous liver metastasis. Liver metastasis is the main cause of death for colorectal patients and the prevention and treatment of colorectal cancer liver metastasis is the top priority to overcome the disease. Active surgical treatment can bring survival benefits to colorectal patients. The authors analyze and summarize the multidisciplinary treatment of colorectal liver metastasis in the department of organ transplantation of First Affiliated Hospital of Kunming Medical University to discuss the hepatectomy strategy of colorectal liver metastasis.
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Objective:To explore the clinical significance of colposcopy in cervical atypical squamous cell of undetermined significance(ASCUS).Methods: Total of 516 women in our department were diagnosed with ASCUS by cytological test(TCT), they were further colposcope test. 209 cases of cervical intraepithelial neoplassia(CIN)were biopsied under the colposcope. In 307 cases of chronic cervicitis were given conservative therapy for 1-3 treatment cycles. Twelve cases of second TCT examination abnormal were biopsied under the colposcope. 295 cases of those with negative cytology checks take TCT regularly.Results: Of the 516 cases, 221 cases of cervical were biopsy (42.82%). Among them, 30 cases were chronic cervicitis , 185 cases were cervical intraepithelial neoplasia (CIN I-III), 6 case were squamous cell carcinoma, respectively. 295 cases without cervical biopsy, the follow-up of 6-18 months TCT found no abnormality.Conclusion: For the patients with cervical ASCUS, colposcopy associate with pathology biopsy may be helpful for the diagnosis and can provide a reasonable and effective treatment scheme for patients.
ABSTRACT
Purpose To investigate the anti-tumor effect of survivin antisense oligonucleotide (Asodn),and whether it can strengthen the sensitivity of chemical therapy with taxol.Methods The experiment was devided into four groups:control group,liposome group,sense oligonucleotide group,and antisense oligonucleotide group.Survivin antisense oligonucleotide was synthesized,and transfected into gastric cancer cells with liposome.The inhibitory rate of proliferation was tested with MTT,and the expression of survivin protein with Western blot;the morphological changes of apoptosis through Hoechst staining were observed, and the apoptosis rate with flow cytometer.Results As observed through Hoechst staining, the cancer cells had normal blue nucleus in the control group, Lip group, and Sodn group, while in Asodn transfection group the nucleus became condensed, with karyorrhexis. The cell inhibitoty rate in Asodn group increased, presenting in a time-dosage dependence manner; survivin protein expression reduced and apoptotic rate increased; there were differences in statistical significance (P<0.05), as compared with the control group, Lip group and Sodn group. The proliferation-inhibitory rate in Asodn plus taxol group [(78.1±0.8) %] was obviously higher than that in Asodn group [(54.9±1.6)%] and taxol group [(56.7%±0.7)%] (P<0.05).Conclusion Survivin antisense oligonucleotide can inhibit the proliferation of gastric cancer cells, induce apoptosis, and strengthen the effect of taxol on the inhibition of tumor growth.